Block Ace powder 免疫阻断剂/封闭剂

Block Ace由牛奶蛋白制备而成，是一种高性能的新型免疫实验阻断剂/封闭剂。由于乳蛋白中包含许多疏水基团，因此它对塑料和膜过滤器具有很高的亲和力。因此，与传统的阻断剂如牛血清白蛋白(BSA)相比，Block Ace阻断剂适合在进行ELISA,Radioimmunoassay (RIA)和Immunoblotting(Western blotting)时,用于阻断抗原或抗体的非特异性结合。

关键特性

由于该产品具有高阻断作用，并将背景维持在低水平，因此可用于ELISA等低浓度检测。

● 经过热处理，牛源抗体、酶和病毒被灭活；

● 在加热和冷冻时是稳定的；

● 能稳定生理活性物质，可作为稀释剂使用；

● 与BSA溶液相比，Block Ace很经济；

● 能在水中迅速溶解。

使用方法

Four (4) grams of Block Ace powder is dissolved into 100 mL of purified water, and this solution is used as a concentrate.

Blocking:

For blocking purpose, either concentrate or 2-4× diluted solution with purified water is used (4× diluted solution is used for ELISA; concentrate is used for Western Blotting).

Dilution:

For dilution of samples and labelled antibody, it is diluted to 10× with purified water.

Washing:

For washing operation such as B/F separation, it is diluted to 10× with purified water, and the required volume of Tween 20 (0.05%–0.2%) is added.

存储方法

Room temperature (it is cold transported)

(This product is heat treated, but there is no preservative or antibiotic. Once dissolved, it should be frozen before storage)

Block Ace与1％BSA溶液之间的封闭效果比较

Anti-human kappa chain antibody and anti-human ramda chain are coated, and it is blocked with either Block Ace or 1% BSA. Human IgG ELISA demonstrates that Block Ace has low background and a steep standard curve.

Block Ace: Blocking reagent还提供其他规格: UK-B500, 500g; UK-B80, 80g