NWLSS™ 谷胱甘肽检测试剂盒是一种基于速率的动力学检测，用于定量包括血液、血浆、组织和培养细胞在内的各种样品中的总 GSH（还原和氧化）。 适用于还原型 GSH 清除后的氧化型 GSH (GSSG) 测量。

推荐用于在组织匀浆、细胞裂解物和其他存在GSH的生物体液中检测GSH或GSSG。该测定适用于所有物种的样品。

Glutathione (GSH, g-glutamylcysteinylglycine), the primary non-protein sulfhydryl in aerobic organisms is synthesized in most cells. The ubiquitous tripeptide is formed by the combination of glutamic acid and cysteine, catalyzed by g-glutamylcysteinyl synthetase. Glycine is then added by glutathione synthetase to form GSH.

In addition to donating an electron during the reduction of hydroperoxides to the respective alcohols (or water in the case of hydrogen peroxide), GSH functions as a co-substrate in the metabolism of xenobiotics catalyzed by glutathione S-transferases. It is also a co-factor for several metabolic enzymes is involved in intracellular transport, functions as an antioxidant and radioprotectant and facilitates protein folding and degradation.

The NWLSS™ Glutathione Assay is a modification of the method first described by Tietze. The general thiol reagent, 5-5'-dithiobis[2-nitrobenzoic acid] (DTNB, Ellman's Reagent) reacts with GSH to form the 412 nm chromophore, 5-thionitrobenzoic acid (TNB) and GS-TNB. The GS-TNB is subsequently reduced by glutathione reductase and β-nicotinamide adenine dinucleotide phosphate (NADPH), releasing a second TMB molecule and recycling the GSH; thus amplifying the response. Any GSSG initially present in the reaction mixture or formed from the mixed disulfide reaction of GSH with GS-TNB is rapidly reduced to GSH and detectable in the assay.

格式：96 tests（1 × 96微孔板），也可用于分光光度计格式比色皿。

用途：总GSH（还原形式和氧化形式）的定量测量

样品要求：全血、血浆、组织和细胞裂解物。

特异性：总谷胱甘肽（GSH + GSSG）

灵敏度：0.1 µM，使用标准方法（全血和一些组织类型）。0.005 µM，采用改进的高灵敏度方法（适用于血浆等GSH水平较低的样品或清除还原的GSH后检测GSSG）。

储存和稳定性：在2-8℃储存时，12个月

Kit Contents:

DTNB Reagent

NADPH

NADPH Diluent

GR Enzyme

GSH (GSSG) Calibrator

Assay Buffer