9(±)-hydroxyoctadecadienoic acid (9± HODE) ELISA Kit

NWLSS™ 9-HODE试剂盒用于定量总9-HODE，其中包括来自任何物种的生物样品中脂质过氧化9(S)-HODE和9(R)-HODE产物。

NWLSS™ 9-HODE ELISA试剂盒的优点：

◆检测9-HODE浓度低至100 pg/mL

◆普遍检测来自任何物种的样品

◆提供另一个有用的脂质过氧化标记物，尤其是在研究多不饱和脂肪酸(PUFAS)的氧化方面

Linoleic acid, the predominant polyunsaturated fatty acid (PUFA) in the human diet, can be metabolized by cyclooxygenase, lipoxygenase and P450 enzymes. The hydroxyoctadecadienoic acid (HODE) derivatives of linoleic acid, 9(R)-HODE, 9(S)-HODE and 13(S)-HODE, are the most widely distributed of the known linoleic acid metabolites. These compounds exhibit interesting biologic activities, including the regulation of platelet function, maintenance of vascular thromboresistance and transduction of the cellular responses to certain growth factors. HODE derivatives may also influence certain pathological states including, psoriasis, the development of atherosclerosis and the development of cancer.

Test Principle

The NWLSS™ 9-HODE kit is a competitive enzyme-linked immuno-assay (ELISA). Briefly, the 9-HODE present in the samples or standards competes with 9(±)-HODE conjugated to horseradish peroxidase [9(±)-HODE-HRP] for binding to an antibody specific for 9(±)-HODE that is precoated on a microplate. The peroxidase activity of 9(±)-HODE-HRP results in color development when a substrate is added. The intensity of the color is proportional to the amount of 9(±)-HODE-HRP bound and is inversely proportional to the amount of unconjugated 9-HODE present in the samples or standards.

格式:96 well竞争ELISA

样品要求:任何物种的血浆、组织匀浆和细胞溶解物。

灵敏度:0.1 ng / mL

用途：定量检测任何物种生物样品中的9(±)-羟基十八碳二烯酸(9± HODE)

储存和稳定性:自生产之日起9个月

Kit Contents:

Microwells precoated with anti-9(±)-HODE: 1 X 96 wells

9(±)-HODE Standard (50 mg/mL in ethanol) 1 X 20 mL

Dilution Buffer (5X) 1 X 25 mL

5X Wash Buffer: 1 X 50 mL

9(±)-HODE-HRP Conjugate (40X): 1 X 320 mL

Reagent Trough: 2 Each