X-Gluc, 5-溴-4-氯-3-吲哚葡萄糖苷
别名:5-溴-4-氯-3-吲哚-β-D-葡糖苷酸环己胺盐;X-GlcA ; 5-Bromo-4-chloro-3-indolyl β-D-glucuronide cyclohexylammonium salt ; X-glucuronide CHA salt。
分子式 C14H13BrClNO7·C6H13N,分子量 521.79,CAS号 114162-64-0,MDL number MFCD00058543。
纯度 ≥98% (TLC);
性状:白色结晶性粉末;
溶于二甲基甲酰胺(DMF),参考浓度10mg/ml。
β-葡糖醛酸酶(β-glucuronidase (GUS))基因检测的显色底物。
X-GlcA is a chromogenic substrate for β-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. X-GlcA is designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype.
储存:−20°C保存。