皮质酮ELISA试剂盒(化学发光)
DetectX®Corticosterone Chemiluminescent ELISA试剂盒定量检测各种样品中存在的皮质酮。

检测类型:Competitive ELISA

样本类型:Serum, Plasma, Urine, Respiratory Vapor, Tissue Culture Media, Fecal Extracts, Feathers, Hair

灵敏度:6.71 pg/mL

适应物种:Corticosterone在不同物种间是相同的

检测时间:2小时

样品数/板:39 in Duplicate

读数:Chemiluminescent

DetectX®皮质酮化学发光ELISA试剂盒用于定量检测血清,血浆,尿液,呼吸蒸汽,粪便提取物,羽毛,毛发和组织培养基中的皮质酮,在进行操作前请阅读完整试剂盒说明书或咨询中昊新生。提供皮质酮标准品以生成标准曲线，将标准品或稀释的样品吸移到包被有抗体的白色微孔板中以捕获绵羊抗体。将皮质酮-过氧化物酶结合物添加到孔内的标准品和样品中，通过向每个孔加入皮质酮绵羊多克隆抗体启动结合反应。孵育两小时后，洗涤板并添加化学发光底物。底物与结合的皮质酮-过氧化物酶结合物反应产生光。

检测原理: 

The Corticosterone Chemiluminescent ELISA Kits quantitatively measure corticosterone in Serum, Plasma, Urine, Respiratory Vapor, Fecal Extracts, Feathers, Hair, and Tissue Culture Media. The Corticosterone Chemiluminescent ELISA Kits are a competitive ELISA. Please read the complete kit insert for more information before performing this assay.

Use our provided corticosterone standard to generate a standard curve for the assay. Pipette the standards or diluted samples into a transparent microtiter plate coated with our donkey anti-sheep IgG antibody. Add the corticosterone peroxidase conjugate and the corticosterone polyclonal sheep antibody. Then incubate the mixture for 2 hours, shaking at room temperature. The immunological reaction occurs between the anti-corticosterone polyclonal antibody, the corticosterone antigen in the sample or standard, and the corticosterone-peroxidase conjugate. As the corticosterone concentration in the sample increases, the bound corticosterone-peroxidase conjugate decreases, causing a decrease in signal and vice versa. 

After the 2-hour incubation, wash away the excess corticosterone-peroxidase and add the chemiluminescent substrate. The chemiluminescent substrate reacts with the bound corticosterone-peroxidase conjugate generating a light detected by a plate reader. Use the intensity and the standard curve to calculate the corticosterone concentration in the samples. 

背景: 

Corticosterone (Kendall’s Compound ‘B’) is a glucocorticoid secreted by the cortex of the adrenal gland. It is produced in the adrenal cortex upon stimulation by ACTH and is the precursor of aldosterone. Corticosterone is a significant indicator of stress and is the prominent stress steroid produced in non-human mammals. Studies involving corticosterone and stress levels have found evidence of impairment of long-term memory retrieval, chronic corticosterone elevation due to dietary restrictions, and corticosterone elevation in response to burn injuries. In addition to stress levels, corticosterone plays a decisive role in sleep-wake patterns.

Arbor Assays为临床重要生物分子研发最高质量的检测和免疫分析试剂盒,在生物领域建立健全的免疫检测和酶活性试剂盒以定量生物分子。Arbor Assays检测试剂盒具有高灵敏性,适用多物种及操作步骤简单的特点。
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