睾酮ELISA试剂盒
DetectX® Testosterone ELISA Kit提供睾酮和双氢睾酮的无创测量。

检测类型: 竞争法ELISA

样本类型: 提取的血清,提取的血浆,粪便提取物,尿液,组织培养基

灵敏度: 9.92 pg/mL

物种: 睾酮在所有物种中相同

检测时间: 2.5小时

样品数/板: 39 in Duplicate

读数: 比色, 450 nm

标准曲线:

DetectX® Testosterone ELISA试剂盒使用特异性抗体检测尿液,粪便或提取的血清和血浆样品中的睾酮及其代谢物（不推荐用于未经提取的血清、血浆或唾液样本）。该睾酮ELISA试剂盒可以定量检测重组缓冲液样品和组织培养基样品中存在的睾酮。

检测原理:
睾酮ELISA试剂盒提供有一个睾酮标准品以生成测定的标准曲线，将标准品或稀释的样品吸移到包被有抗体的透微孔板中以捕获兔抗体。将睾酮-过氧化物酶偶联物添加到孔中，通过向每个孔中加入睾酮多克隆抗体来引发结合反应。孵育2小时后，洗涤板并加入底物。底物与结合的睾酮-过氧化物酶结合物反应。短暂孵育后，停止反应，并在一个可测量450nm波长的微量滴定板阅读器中检测产生的颜色的强度。

The DetectX® Testosterone ELISA Kit uses a specifically generated antibody to measure testosterone and its metabolites in urine and fecal samples, or in extracted serum and plasma. The kit will quantitatively measure Testosterone present in reconstituted buffer samples and tissue culture media samples. Please read the complete kit insert before performing this assay.

背景:
Testosterone (4-Androsten-17β-ol-3-one) is an anabolic steroid hormone from the androgen group. It is found in mammals, reptiles, birds, and other vertebrates. In mammals, testosterone is primarily secreted in the testes of males and the ovaries of females, although small amounts are also secreted by the adrenal glands.

Testosterone is the principal male sex hormone and plays key roles in the development of reproductive tissues such as the testes and prostate. It also promotes secondary sexual characteristics such as increased muscle, bone mass, and body hair. Testosterone plays a significant role in glucose homeostasis, lipid metabolism and the prevention of osteoporosis. Cross-sectional epidemiological studies have reported a direct correlation between plasma testosterone and insulin sensitivity. Low testosterone levels are associated with an increased risk of type 2 diabetes, dramatically illustrated by androgen deprivation in men with prostate carcinoma.

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