Cyclic GMP Direct ELISA试剂盒

DetectX®cGMP Direct ELISA试剂盒直接定量检测裂解细胞,血浆,尿液,唾液和培养基样品中的cGMP。

检测类型:

Competitive ELISA

样本类型:

Cell Lysates, Urine, Plasma, Saliva, Tissue Culture Media

灵敏度:

0.28 pmol/mL (Regular), 0.188 pmol/mL (Acetylated)

适应物种:

Cyclic GMP在不同物种间是相同的

检测时间:

2.5小时

样品数/板:

39 in Duplicate

读数:

Colorimetric, 450 nm

DetectX®Cyclic GMP Direct ELISA试剂盒是多格式的，可以检测各种样品类型中的乙酰化和非乙酰化cGMP。该试剂盒独特之处在于所有的样品和标准品均被稀释成酸性的样品稀释剂，其中包含特殊的添加剂和稳定剂，用于裂解细胞进行测量。 结合反应需要2个小时完成。 洗涤板并加入底物。 底物发生反应以生成颜色，颜色停止后，读取生成的颜色的强度。

Cyclic GMP is a critical and multifunctional second messenger present at levels typically 10-100 fold lower than cAMP in most tissues. Intracellular cGMP is formed by the action of the enzyme guanylate cyclase on GTP and degraded through phosphodiesterase hydrolysis. Guanylate cyclases (GC) are either soluble or membrane bound. Soluble GCs are nitric oxide responsive, whereas membrane bound GCs respond to hormones such as acetylcholine, insulin and oxytocin. Other chemicals like serotonin and histamine also cause an increase in cGMP levels. Cyclic GMP regulates cellular composition through cGMP-dependent kinase, cGMP-dependent ion channels or transporters, and through its hydrolytic degradation by phosphodiesterase.

This kit is multi-format and can detect both acetylated and non-acetylated cGMP in a variety of sample types.

Arbor Assays为临床重要生物分子研发最高质量的检测和免疫分析试剂盒,在生物领域建立健全的免疫检测和酶活性试剂盒以定量生物分子。Arbor Assays检测试剂盒具有高灵敏性,适用多物种及操作步骤简单的特点。

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