The DetectX® Oxytocin ELISA Kit is designed to quantitatively measure Oxytocin present in serum, plasma, clarified milk and tissue culture media samples.

检测类型: Competitive ELISA
样本类型: Serum, Plasma (EDTA and Heparin), Saliva, Clarified Milk, Tissue Culture Media
灵敏度: 17.0 pg/mL
适用物种: Oxytocin在不同物种间是相同的
检测时间: Overnight Assay (16-18小时)
样品数/板: 38 in Duplicate
读数: Colorimetric, 450 nm

检测原理:
The Oxytocin ELISA Kit quantitatively measures oxytocin in Serum, Plasma (EDTA and Heparin), Saliva, Clarified Milk, and Tissue Culture Media. The Oxytocin ELISA Kits is a competitive ELISA. Please read the complete kit insert for more information before performing this assay.

Use our provided oxytocin standard to generate a standard curve for the assay. Pipette the standards or diluted samples into a transparent microtiter plate coated with our goat anti-rabbit IgG antibody. Add the oxytocin peroxidase conjugate and the oxytocin polyclonal rabbit antibody. Then incubate the mixture overnight, shaking at 4C degrees. The immunological reaction occurs between the anti-oxytocin polyclonal antibody, the oxytocin antigen in the sample or standard, and the oxytocin-peroxidase conjugate. As the oxytocin concentration in the sample increases, the bound oxytocin-peroxidase conjugate decreases, causing a decrease in signal and vice versa.

After the overnight incubation, wash away the excess oxytocin-peroxidase and add the TMB substrate. The TMB substrate reacts with the bound cortisol-peroxidase conjugate generating a signal detected by a plate reader at 450nm. Use the intensity and the standard curve to calculate the corticosterone concentration in the samples.

背景:
The neuropeptides oxytocin and vasopressin were isolated and synthesized by Vincent du Vigneaud, work for which he received the Nobel Prize in Chemistry in 1955. The molecule consists of nine amino acids linked with a [1-6] disulfide bond and a semi-flexible carboxyamidated tail. Highly conserved across species boundaries, oxytocin-like neurohypophysial peptides are substituted primarily at residues 4 &/or 8. In the oxytocin-like peptide, mesotocin; (a common peptide found in some fishes, reptiles, amphibians, marsupials, and non-mammalian tetrapods), there is isoleucine at residue eight instead of leucine.

Oxytocin is a neurohypophysial peptide produced in the hypothalamus. It binds to specific cell surface receptors, initiating a secondary intracellular response cascade via a phosphoinositide signaling pathway. Oxytocin is also commonly known as the “love hormone” because it plays essential roles in many reproduction-related functions in both males and females. Oxytocin functions in behavioral actions such as orgasm, social recognition, pair bonding, anxiety, and maternal behaviors. Other classical activities of oxytocin include stimulation of uterine smooth muscle contraction during labor and milk ejection during lactation.