Urea Nitrogen Assay Kit,尿素氮检测试剂盒

DetectX®尿素氮(BUN)检测试剂盒可在30分钟内定量检测Urea Nitrogen。

检测类型:Detection Kit

样本类型:Serum, Plasma, Urine, Saliva

灵敏度:30 µg/dL

适应物种:人

检测时间:30分钟

样品数/板:40 in Duplicate

读数:Colorimetric, 450 nm

尿素氮(BUN)检测试剂盒定量检测血清,血浆,尿液和唾液中的尿素氮。在进行此分析之前，请阅读完整的试剂盒说明书。尿素氮(BUN)检测试剂盒可在30分钟内完成检测。

检测原理:
Use our provided urea nitrogen standard to generate a standard curve for the assay. Pipette the standards or diluted samples into a transparent microtiter plate, along with Color Reagents A and B. We recommend a 30-minute incubation shaking at room temperature, and after 30 minutes, use a plate reader at 450nm to detect the resulting signal. Use the recorded intensity and the standard curve to calculate the urea nitrogen concentration in the samples. 

背景:

Urea is a by-product of protein metabolism by the liver and is removed from the blood by the kidneys. Urea freely filters through the glomerulus but is reabsorbed by the renal tubules in a flow-dependent fashion. A higher flow rate causes more urea nitrogen to be cleared from the circulation and eliminated through the kidneys. As a result, the level of circulating urea nitrogen and serum creatinine serves as a primary measure of kidney function.

Average adult blood urea nitrogen (BUN) levels should be between 7 and 21 mg urea nitrogen per 100 mL blood (mg/dL). Azotemia, poor kidney function, will cause elevated BUN levels (≥ 50 mg/dL) and is associated with acute kidney failure or injury, severe acute pancreatitis, congestive heart failure, or gastrointestinal bleeding. Azotemia also can occur with dehydration, as a result of alcohol abuse, or with high protein diets. Lower than expected BUN levels are usually not clinically predictive but are primarily associated with liver disease or malnutrition, including malabsorption and low protein diets. Urine and saliva are acceptable non-invasive samples for the measurement of urea nitrogen.

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