人脑钠素ELISA试剂盒,人BNP ELISA试剂盒

DetectX®人B-Type Natriuretic Peptide (BNP) ELISA试剂盒用于定量检测血清和血浆样本中的BNP。

检测类型:Sandwich ELISA

样本类型:Serum and EDTA Plasma

灵敏度:0.31 pg/mL

适应物种:Human

检测时间:2.5 Hours

样品数/Kit:40 in Duplicate

读数:Colorimetric, 450 nm

检测原理:

The Human B-Type Natriuretic Peptide (BNP) ELISA Kit quantitatively measures BNP in serum and EDTA plasma. The Human B-Type Natriuretic Peptide (BNP) ELISA Kit is a sandwich ELISA with a run time of 2.5 hours. Please read the complete kit insert for more information before performing this assay.

Use our provided BNP standard to generate a standard curve for the assay. Pipette the standards or diluted samples into a transparent microtiter plate coated with our sheep anti-human antibody. Add the peroxidase-conjugated BNP monoclonal antibody. Then incubate the mixture covered at room temperature for 2 hours. The immunological reaction occurs between the peroxidase-conjugated BNP monoclonal antibody and the BNP antigen in the sample or standard. 

After the 2-hour incubation, wash away the excess peroxidase-conjugated BNP monoclonal antibody and add the TMB substrate. The TMB substrate reacts with the bound peroxidase-conjugated BNP monoclonal antibody generating a signal detected by a plate reader at 450nm. Use the intensity and the standard curve to calculate the BNP concentration in the samples.

背景:
B-Type Natriuretic Peptide (BNP [formerly known as brain natriuretic peptide]) is an important hormone that regulates blood pressure and volume. In patients with heart failure, increased heart stretch or hypoxia can trigger BNP synthesis and secretion from ventricular cardiomyocytes, as opposed to its standard constitutive release from only the atrium. Upon secretion, proBNP is cleaved into active BNP (32 amino acids), having a half-life of 20 minutes, and inactive NT-proBNP (76 amino acids), having a half-life of 90 minutes. Patients suffering from heart failure and cardiac dysfunction have significantly elevated BNP serum levels, so this biomarker is an excellent addition to the diagnostic criteria for these diseases. Patients with serum concentrations greater than 100 pg/mL of BNP are likely to suffer from heart failure or cardiac dysfunction. Not only can you determine the diagnostic outcome from this biomarker, but you can also monitor treatment efficacy for patients suffering from heart failure or cardiac dysfunction

____________________________________________________________

Arbor Assays为临床重要生物分子研发最高质量的检测和免疫分析试剂盒,在生物领域建立健全的免疫检测和酶活性试剂盒以定量生物分子。Arbor Assays检测试剂盒具有高灵敏性,适用多物种及操作步骤简单的特点。
北京中昊新生科技作为美国Arbor Assays中国代理,一直为国内客户提供及时高效的配送和更专业的产品技术支持。为您提供人BNP ELISA试剂盒,,人脑钠素ELISA试剂盒最新现货优惠信息。