催产素ELISA试剂盒(化学发光), Oxytocin CLIA Kit
DetectX®Oxytocin Chemiluminescent ELISA试剂盒用于定量检测血清,血浆,澄清乳和组织培养基样品中的催产素。

检测类型:Competitive ELISA

样本类型:Serum, Plasma, Saliva, Clarified Milk, Tissue Culture Media

灵敏度:6.33 pg/mL

适应物种:Oxytocin在不同物种间是相同的

检测时间:Overnight Assay (16-18小时)

样品数/板:38 in Duplicate

读数:Chemiluminescent

DetectX^®催产素ELISA试剂盒(化学发光)提供催产素标准品以生成测定的标准曲线，并且应从标准曲线中读取所有样品。将标准品或稀释的样品吸移到涂有抗体的白色微孔板中以捕获兔抗体。将催产素-过氧化物酶结合物添加到孔中的标准品和样品中，通过向每个孔中添加催产素多克隆抗体开始结合反应。在4℃下孵育过夜后，洗板并加入化学发光底物。底物与结合的催产素-过氧化物酶结合物反应产生光，在具有测量发光功能的微孔板读板仪中检测产生的化学发光信号的强度。在对样品的稀释进行适当的校正后，使用大多数读板仪提供的软件计算样品中催产素的浓度。
             

The Oxytocin Chemiluminescent ELISA Kits quantitatively measure oxytocin in Serum, Plasma, Saliva, Clarified Milk, and Tissue Culture Media. The Oxytocin Chemiluminescent ELISA Kits are competitive ELISAs. Please read the complete kit insert for more information before performing this assay.

Use our provided oxytocin standard to generate a standard curve for the assay. Pipette the standards or diluted samples into a transparent microtiter plate coated with our goat anti-rabbit IgG antibody. Add the oxytocin peroxidase conjugate and the oxytocin polyclonal rabbit antibody. Then incubate the mixture overnight, shaking at 4C degrees. The immunological reaction occurs between the anti-oxytocin polyclonal antibody, the oxytocin antigen in the sample or standard, and the oxytocin-peroxidase conjugate. As the oxytocin concentration in the sample increases, the bound oxytocin-peroxidase conjugate decreases, causing a decrease in signal and vice versa. 

After the overnight incubation, wash away the excess oxytocin-peroxidase and add the chemiluminescent substrate. The chemiluminescent substrate reacts with the bound corticosterone-peroxidase conjugate generating a light detected by a plate reader. Use the intensity and the standard curve to calculate the corticosterone concentration in the samples.

背景:

The neuropeptides oxytocin and vasopressin were isolated and synthesized by Vincent du Vigneaud, work for which he received the Nobel Prize in Chemistry in 1955. The molecule consists of nine amino acids linked with a [1-6] disulfide bond and a semi-flexible carboxyamidated tail. Highly conserved across species boundaries, oxytocin-like neurohypophysial peptides are substituted primarily at residues 4 &/or 8. In the oxytocin-like peptide, mesotocin; (a common peptide found in some fishes, reptiles, amphibians, marsupials, and non-mammalian tetrapods), there is isoleucine at residue eight instead of leucine. 

Oxytocin is a neurohypophysial peptide produced in the hypothalamus. It binds to specific cell surface receptors, initiating a secondary intracellular response cascade via a phosphoinositide signaling pathway. Oxytocin is also commonly known as the “love hormone” because it plays essential roles in many reproduction-related functions in both males and females. Oxytocin functions in behavioral actions such as orgasm, social recognition, pair bonding, anxiety, and maternal behaviors. Other classical activities of oxytocin include stimulation of uterine smooth muscle contraction during labor and milk ejection during lactation.

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Arbor Assays为临床重要生物分子研发最高质量的检测和免疫分析试剂盒,在生物领域建立健全的免疫检测和酶活性试剂盒以定量生物分子。Arbor Assays检测试剂盒具有高灵敏性,适用多物种及操作步骤简单的特点。

北京中昊新生科技作为美国Arbor Assays中国代理,一直为国内客户提供及时高效的配送和更专业的产品技术支持。为您提供催产素ELISA试剂盒,Oxytocin CLIA Kit最新现货优惠信息。