醛固酮ELISA试剂盒
DetectX®Aldosterone ELISA试剂盒用于检测提取的血清和血浆,尿液,唾液,提取的干燥粪便样本和组织培养基样本中存在的醛固酮。
检测类型:
Competitive ELISA
样本类型:
Extracted Serum, Extracted Plasma (EDTA and Heparin), Urine, Saliva, Fecal Extracts, Tissue Culture Media
灵敏度:
4.97 pg/mL
适应物种:
Aldosterone在不同物种间是相同的
检测时间:
2.5小时 or Overnight
样品数/板:
40 in Duplicate
读数:
Colorimetric, 450 nm
DetectX®醛固酮酶免疫测定试剂盒用于定量测量提取的血清和血浆、尿液、提取的干燥粪便样品和组织培养液样品中的醛固酮。 提供了醛固酮储备液以生成标准曲线;提供有制备4,000至3.906 pg/mL或5,000至8.192 pg/mL的测定标准液的方案。 将样品吸移到涂有抗体的透明微孔板中以捕获绵羊抗体。 将醛固酮-过氧化物酶结合物加入标准品和样品中。 通过向每个孔添加针对醛固酮的多克隆抗体来引发结合反应。 孵育后,洗涤板并加入底物。 底物与结合的醛固酮-过氧化物酶结合物反应。 短暂孵育后,停止反应,并在450nm处检测到所产生颜色的强度。
Aldosterone is a mineralocorticoid first isolated by the husband and wife team of Simpson and Tait at University College, London. Aldosterone controls the sodium-potassium balance through unidirectional salt reabsorption in a variety of tissues and glands. Synthesized from cholesterol in the zona glomerulosa of the adrenal cortex, secretion is regulated through the renin-angiotensin system. Angiotensin II and potassium stimulate primary secretion by increasing the rate of production of the steroid. Peripheral aldosterone levels are dependent on age and body position. Aldosterone measurement is useful in the investigation of primary and secondary aldosteronism including vascular disease, salt depletion, potassium loading, cardiac failure with ascites, and pregnancy.
Arbor Assays为临床重要生物分子研发最高质量的检测和免疫分析试剂盒,在生物领域建立健全的免疫检测和酶活性试剂盒以定量生物分子。Arbor Assays检测试剂盒具有高灵敏性,适用多物种及操作步骤简单的特点。
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