DetectX®人ST2 ELISA试剂盒用于定量检测各种样品和组织培养基中存在的ST2。
检测类型:Sandwich ELISA
样本类型:Serum, Plasma, Tissue Culture Media
灵敏度:30.5 pg/mL
适应物种:人
检测时间:3小时
样品数/板:40 in Duplicate
读数:Colorimetric, 450 nm
DetectX®人ST2 ELISA试剂盒提供有重组人ST2标准品以产生测定的标准曲线,将标准品或稀释的样品移取到包被有单克隆抗体的微孔板中以捕获ST2。孵育60分钟后洗板。加入生物素化的ST2抗体,再次将板孵育60分钟。第二次洗涤后,加入过氧化物酶缀合的链霉亲和素,孵育30分钟并洗涤。然后加入底物,它与结合过氧化物酶缀合的链霉亲和素反应。孵化后,所生成的颜色的强度在450nm处读取。执行试剂盒操作前请阅读详细说明书或咨询中昊新生(zhscience.com)。
ST2(也称为生长刺激表达基因2,IL1RL1,DER4,T1和FIT-1)是Toll样/IL-1受体超家族的成员。临床和实验观察显示ST2与哮喘、肺纤维化、类风湿性关节炎、胶原血管疾病和感染性休克等疾病相关。临床上,急性支气管哮喘患者的ST2血清水平升高,呼吸急促的急诊室患者血清水平也会升高。ST2的血清水平可以区分心力衰竭和非心血管病因。
ST2 (also known as IL1RL1, DER4, T1 and FIT-1) is a member of the Toll-like/IL-1-receptor superfamily. The interleukin-1 (IL-1) receptor family has several members, including the classical interleukin-1 receptor (IL-1R) and the interleukin-18 receptor (IL-18R). In 1989, one member of the family, ST2, was identified as an orphan receptor. Investigation into the function of ST2 revealed its participation in inflammatory processes, particularly regarding mast cells, type 2 CD4+ T-helper cells, and the production of Th2-associated cytokines. ST2 was characterized as a specific cellular marker that differentiated Th2 from Th1 T-cells. The gene for ST2 spans ~40 kb on human chromosome 2q12, and is part of the larger human IL-1 gene cluster of ~200 kb. ST2 is conserved across species, with homologues in the genomes of mouse, rat, and fruitfly. The ~37 kDa unglycosylated secreted protein is converted into a 60–70 kDa glycosylated product, which is the soluble form of ST2, sST2.
Arbor Assays为临床重要生物分子研发最高质量的检测和免疫分析试剂盒,在生物领域建立健全的免疫检测和酶活性试剂盒以定量生物分子。Arbor Assays检测试剂盒具有高灵敏性,适用多物种及操作步骤简单的特点。
北京中昊新生科技作为美国Arbor Assays中国代理,一直为国内客户提供及时高效的配送和更专业的产品技术支持。为您提供人ST2 ELISA试剂盒,人IL1RL1 ELISA试剂盒最新现货优惠信息。