人髓过氧化物酶ELISA试剂盒

DetectX®人Myeloperoxidase(MPO) ELISA试剂盒用于定量检测各种基质中存在的MPO。

检测类型:Sandwich ELISA

样本类型:Serum, Platelet-Poor Heparin Plasma, Saliva, Urine, Tissue Culture Media

灵敏度:68 pg/mL

适应物种:人

检测时间:2.5小时

样品数/板:40 in duplicate

读数:Colorimetric, 450 nm

人髓过氧化物酶ELISA试剂盒可以检测血清,乏血小板肝素血浆,唾液,尿液,组织培养基样品,试剂盒提供有髓过氧化物酶标准品以生成测定的标准曲线，并且应从标准曲线中读取所有样品。将标准品或稀释的样品吸移到包被有单克隆抗体的微孔板中，以捕获样品中存在的MPO。孵育60分钟后，将板洗涤。加入过氧化物酶偶联的MPO抗体，并将板再次孵育60分钟并洗涤。然后将底物添加到平板中，使其与结合的MPO偶联抗体反应。第三次孵育后，停止反应，并在能够测量450 nm波长的微量滴定板读取器中检测所产生颜色的强度。使用适用于大多数读板器的软件进行适当的稀释校正后，即可计算样品中髓过氧化物酶的浓度。进行测定之前，请阅读完整的试剂盒说明书或咨询中昊新生(zhscience.com)。

Myeloperoxidase (MPO) is a tetrameric heme-containing protein abundantly produced in neutrophil granulocytes where it plays an important anti-microbial role. Normally stored in azurophilic granules, MPO is released into the extracellular space during degranulation. There, as part of the neutrophils “respiratory burst”, it produces hypochlorous acid from hydrogen peroxide and Cl- . MPO also uses hydrogen peroxide to oxidize tyrosine to the tyrosyl radical. Both hypochlorous acid and tyrosyl are cytotoxic and when present can kill bacteria and other pathogens. Hereditary deficiency of myeloperoxidase predisposes individuals to immune deficiency.

Studies have shown an association between elevated MPO levels and coronary artery disease, and in 2003 it was suggested that MPO may serve as a sensitive predictor of myocardial infarction in patients complaining of chest pain. Since that time the clinical utility of MPO testing in cardiac patients has been solidly established in the literature with well over 100 papers published. In 2010 this clinical application was further refined by additional studies which determined that measuring both MPO and C-reactive protein (CRP) provided more accurate prediction of mortality risk than measuring just CRP alone.

Arbor Assays为临床重要生物分子研发最高质量的检测和免疫分析试剂盒,在生物领域建立健全的免疫检测和酶活性试剂盒以定量生物分子。Arbor Assays检测试剂盒具有高灵敏性,适用多物种及操作步骤简单的特点。

北京中昊新生科技作为美国Arbor Assays中国代理,一直为国内客户提供及时高效的配送和更专业的产品技术支持。为您提供人Myeloperoxidase(MPO) ELISA试剂盒,人髓过氧化物酶ELISA试剂盒最新现货优惠信息。