硝基酪氨酸(NTR) ELISA试剂盒推荐用于检测血浆或血清样品中的亚硝基化蛋白。 作为一种改进的测量单取代硝基酪氨酸(NTR)蛋白加合物的方法，该ELISA试剂盒已针对具有单个NTR取代的标准进行了优化。
相比之下，我们最初的夹心版本Nitrotyrosine(NTR) ELISA（NWK-NTR01-01/NWK-NTR01-02）采用每个分子有两个或多个取代的标准。虽然这两种方法都可以检测硝基酪氨酸蛋白加合物，但预计使用新的竞争法ELISA可能更容易检测到由于较低水平的亚硝化应激引起的修饰。

Nitrate (NO₃) and nitrite (NO₂) production are typically used as markers of nitric oxide production (NO) production. These analytes by themselves fail to address the eventual fate of NO or the possible adverse effects associated with its excess production and reaction with free radical species in vivo. Active NO metabolites can react with superoxide to form peroxynitrite (ONOO-) a powerful oxidant and nitrating agent. Subsequent reaction of peroxynitrite with proteins results in nitrotyrosine formation.

As a stable end product of peroxynitrite mediated oxidation/nitration, Nitrotyrosine can be used as a surrogate index of NO dependent damage in vivo and has been associated with multiple disease states.

This assay features a “competitive” ELISA format in which standards and samples are first incubated with a biotynylated Tracer Antibody in a separate U-bottom microplate. After incubation, the standards and samples are transferred to a microplate precoated with nitrated-HSA and allowed to incubate for an additional hour.

During this step unbound Biotinylated Tracer Antibody binds to stationary phase NO-HAS. A subsequent wash removes excess Tracer and Tracer/protein complex. Addition of streptavidin-peroxidase followed by tetramethylbenzidine (TMB) facilitates color development inversely proportional to the nitrotyrosine present in the sample. The reaction is stopped using an oxalic acid solution and the assay is read on a plate reader at 450 nm.

格式:96孔可拆酶标板

样品要求:血浆、血清和其他生物样品，如组织匀浆样品。

特异性:亚硝基蛋白加合物

灵敏度:50 nm单价硝基酪氨酸(单修饰每个分子)

储存和稳定性:在规定温度下储存，自生产之日起1年

试剂盒组分:

Precoted Microwell strips and strip frames

Wash Buffer

Dilution Buffer

Nitrotyrosine Standard

Biotinylated anti nitrotyrosine tracer

Streptavidin Peroxidase

TMB Substrate

Stop Solution