N-乙基马来酰亚胺(NEM)/硫醇掩蔽剂
产品NWR-NEM01，N-乙基马来酰亚胺(NEM)，巯基掩蔽剂，烷基化自由巯基的试剂。在检测氧化型谷胱甘肽 (GSSG) 时，它可用于使含有还原型谷胱甘肽 (GSH) 的硫醇不再反应。 当按照指示使用时，该产品提供足够的NEM来处理100个样品，为使用NWLSS™ NWK-GSSG01检测试剂盒或类似产品类型的GSSG检测做准备。
N-乙基马来酰亚胺(NEM)/硫醇掩蔽剂兼容所有物种的样品。
Rapid oxidation of GSH in non-reducing environments (e.g., outside of cells) presents a very real obstacle to accurate measurement of GSSG. Proper masking of reduced GSH requires immediate (if possible) treatment of samples with NEM in approximate 10 fold molar excess compared to expected sample GSH. Failure to treat samples in a timely manner allows for artifact GSH oxidation to occur leading to overestimation of GSSG.
Test Principle
If using with NWLSS™ GSSG or NWLSS™ GSHt/GSSG Assay kits, sample specific instructions for use can be found in the associated product inserts. Note: Excess NEM must be removed by extraction prior to assaying for GSSG.
格式：NEM试剂加稀释剂
预期用途：推荐作为有效的硫醇掩蔽剂
样品要求：与全血、组织匀浆、RBC和其他细胞裂解物一起使用时有效。
特异性：巯基（硫醇）掩蔽剂
储存和稳定性：2-8℃储存，12个月
试剂盒组分：
NEM试剂：1 x 60 mg
NEM稀释剂：1 x 2 mL
400-888-0207
