For setup and optimization of miRNA quantification experiments using SYBR® Green-based qPCR

Features

Easy and cost-effective introduction to the most sensitive miRNA qPCR system available

No false positives, just true miRNA signals

Includes RNA spike-in template and control primer set for monitoring performance

Contains everything you need to perform small-scale experiments

Fast and very easy protocol

Product Details

GeneRead DNAseq Gene Panels能够对特定的一组基因进行可靠的遗传变化分析，适用于二代测序应用。该产品提供的多重PCR引物用于扩增研究所需的一组基因或与某种疾病相关的基因的外显子。GeneRead DNAseq Gene Panels还可依据您选择的基因提供定制产品，以满足您的实验需求。高品质的引物设计和靶向扩增试剂确保扩增的位点覆盖全面、无偏差、特异性高，保留您珍贵样本中即便是少量的遗传变异信息。

Performance

Unmatched sensitivity

Universal reverse transcription combined with LNA-enhanced and Tm-normalized primers enables accurate and reliable quantification of individual miRNAs from as little as 1 pg total RNA . With exceptional sensitivity and extremely low background, you can accurately quantify very low levels of miRNA without pre-amplification. This makes the miRCURY LNA miRNA PCR System suitable for all types of samples, especially those with low RNA content, such as serum, plasma and other biofluids.

Principle

High-performance reagents for quantitative, real-time RT-PCR

miRCURY LNA RT Kit

This kit includes all the necessary reagents to perform fast and convenient miRNA polyadenylation and reverse transcription in a single reaction step. Using a single, universal RT reaction as the template for subsequent PCR amplifications greatly simplifies the procedure compared to systems that require miRNA-specific, first-strand synthesis. The UniSp6 RNA spike-in can be used with the control assay – UniSp6 miRCURY LNA miRNA PCR Assay (cat. no. YP00203954) – as a reverse transcription positive control and/or as an inter-plate calibrator in our miRNA PCR panels.

miRCURY LNA SYBR Green PCR Kit

This kit contains the high-performance miRCURY SYBR Green Master Mix for quantitative real-time PCR amplification. The detection is based on SYBR Green, which allows quality control of the resulting PCR amplicon by melting curve analysis. LNA-enhanced primer sets require specific PCR reaction conditions for optimal performance. The kit also includes a control primer set designed for amplification of the synthetic RNA spike-in provided in the miRCURY LNA RT Kit. Refer to the miRCURY LNA RT Kit Handbook for details. The miRCURY LNA miRNA PCR Assays and Panels have been validated using the miRCURY SYBR Green Master Mix; use of other reagents for qPCR will affect the results.

Procedure

The miRCURY LNA miRNA PCR Assay system is an miRNA-specific, LNA-based system designed for sensitive and accurate detection of miRNA by quantitative real-time PCR using SYBR Green. The first step of the procedure is universal reverse transcription, followed by real-time PCR amplification with LNA-enhanced primers.

Applications

The mirCURY LNA miRNA PCR Starter Kit is used as part of the miRCURY PCR System for:

Setup of miRNA quantification experiments

miRNA control experiments