NWLSS™人过氧化还原酶3 ELISA试剂盒用于体外定量检测人血清、血浆、细胞裂解物和其他生物样品中的Peroxiredoxin 3。该检测识别天然和重组人Peroxiredoxin 3。

Mammals have 6 isoforms of the anti oxidative protein Peroxiredoxin (Prx). The specific localization of Prx3 in mitochondria together with the electron donors, thioredoxin 2 and thioredoxin reductase 2, suggest that these three proteins may provide a primary line of defense against H2O2 produced by the mitochondrial respiratory chain. Organisms living under aerobic conditions have developed various anti-oxidative mechanisms to protect them from damage by reactive oxygen species (ROS). Peroxiredoxin (Prx) is a growing anti-oxidative protein family that has been identified six members in mammals. They share a common reactive Cys residue in the N-terminal region, and are capable of serving as a peroxidase and involve thioredoxin and/or glutathione as the electron donor. Prx1 to Prx4 have an additional Cys residue in the conserved C-terminal region, and are cross members as judged by the amino acid sequence similarity. Prx5 also contains an additional Cys in its C-terminal region that is less conserved. On the other hand, Prx6 has only one unique Cys. These Prx family members are distributed in subcellular localization, Prx1, 2, and 6 in cytosol, Prx3 in mitochondria, Prx4 in ER and secretion, Prx5 showing complicated distribution including peroxisome, mitochondria and cytosol, all of which are potential sites of ROS production. In addition to their role as a peroxidase, however, a body of evidence has accumulated to suggest that individual members also serve divergent functions, which are associated with various biological processes such as the detoxification of oxidants, cell proliferation, differentiation and gene expression. It would be expected that these functions might not necessarily depend on peroxidase activity and, therefore, it seems likely that the divergence is due to unique molecular characteristics intrinsic to each member. The specific localization of Prx3 in mitochondria together with the identification of its mitochondria-specific electron suppliers, namely thioredoxin 2 and thioredoxin reductase 2, suggest that these three proteins might provide a primary line of defense against H2O2 produced by the mitochondrial respiratory chain. Furthermore, Prx3 expression is induced by oxidants in the cardiovascular system and is thought to play a role in the antioxidant defense system and homeostasis within mitochondria.

检测原理

The NWLSS™ Peroxiredoxin 3 (Prx3) ELISA kit is to be used for the in vitro quantitative determination of human Prx3 in serum, plasma, cell lysate and other biological samples. The assay will recognize both native and recombinant human Prx3.

别名：PRDX3;Peroxiredoxin 3; MER5; AOP-1; AOP1; SP-22; Antioxidant Protein 1; Thioredoxin-dependent peroxide reductase, mitochondrial; 过氧化还原酶3

格式：96孔可拆酶标板

预期用途：定量测量生物样品中人Peroxiredoxin 3。

样品要求：可能存在过氧化还原酶3的人血浆和其他生物体液

特异性：人过氧化还原酶3

灵敏度：2 ng/mL 至 32 ng/mL

储存和稳定性：在指定温度下储存，自生产日期起1年

Kit Contents:

Microwell strips precoated w/anti huPrx3 and strip frame

rHu-Peroxiredoxin 3 Standard (lyophilized)

Sample Dilution Buffer

100X Secondary Antibody (biotin labeled anti-huPrx1)

Reagent Dilution Buffer

100X Avidin-HRP Conjugate

Assay Preparation Buffer

TMB Substrate

Stop Solution

10X Concentrated Wash Buffer

Adhesive Plate Covers