NWLSS™ 8-Isoprostane (15-Isoprostane F2t) ELISA Kit for Plasma & Tissue

8-异构前列腺素（15-异构前列腺素F2t）ELISA试剂盒 用于血浆和组织样品

8-异前列腺素(15-异前列腺素F2t) ELISA试剂盒用于定量检测8-Isoprostane (15-isoprostane F2t)，也称为8-epi-PGF2a 和 8-iso-PGF2a、8-异构前列腺素F2α。 这种酶联免疫吸附检测可用于在固相萃取含异前列腺素的部分后，检测血清和组织样品。

NWLSS™ 8-异前列腺素（15-异前列腺素F2t）ELISA的优势：

◆检测低至50 pg /mL

◆对8-Isoprostane (15-Isoprostane F2t) 具有高度特异性：对9α,11β-Prostaglandin F2α、Prostaglandin F2α、6-Keto-Prostaglandin F1α、Prostaglandin E2、Prostaglandin D2 或Arachidonic Acid检测结果是低或无交叉反应性

◆还提供磷脂水解后总8-异构前列腺素（15-异构前列腺素F2t）的定量

Isoprostanes are prostaglandin-like compounds that are produced by free radical mediated peroxidation of lipoproteins. Levels of 8-Isoprostane (15-isoprostane F2t), a representative isoprostane in biological fluids, have been shown to be useful for assessment of oxidant stress in vivo. 8-Isoprostane (15-isoprostane F2t) has also been shown to be a potent vasoconstrictor in rat kidneys and rabbit lungs and may play a causative role in atherogenesis. Elevated 8-isoprostane (15-isoprostane F2t) levels are associated with hepatorenal syndrome, rheumatoid arthritis, athero-sclerosis, and carcinogenesis.

Test Principle

This assay utilizes a competitive enzyme-linked immunoassay (ELISA) format. Briefly, 8-Isoprostane (15-isoprostane F2t) in the samples or standards is allowed to compete with 8-Isoprostane (15-isoprostane F2t) conjugated to horseradish peroxidase (HRP) for binding to a polyclonal antibody specific for 8-Isoprostane (15-isoprostane F2t) coated on a microplate. Subsequent TMB substrate addition results in a blue color development that is inversely proportional to the quantity of 8-Isoprostane (15-isoprostane F2t) in the original samples or standards. Addition of an acid stop solution causes a color change to yellow where absorbance is read at 450 nM.

Format: 96 Well Competitive ELISA

Sample Requirements:Urine, serum or tissue samples from any species

Specificity:

(100.0%) 8-Isoprostane (15-isoprostane F2t)

(4.1%) 9α,11β-Prostaglandin F2α

(3.0%) 13,14-Dihydro-15-Keto-PGF2α

(<0.01%) 9α,11β-Prostaglandin F2α

(<0.01%) Prostaglandin F2α

(<0.01%) 6-Keto-Prostaglandin F1α

(<0.01%) Prostaglandin E2

(<0.01%) Prostaglandin D2

(<0.01%) Arachidonic Acid

Sensitivity:Lower Limit of Detection (LLD)= 0.005 pg/mL

Effective Range:0.05 ng/mL - 10 ng/mL 

Note that although the analyte is detectable at levels below 50 pg/mL, data within the effective range of this assay will yield greater reproducibility. Investigators may wish to repeat samples with data below this range for added confidence.

Storage and Stability:9 months from the date of manufacture

Kit Contents:

96-well microtiter plate, pre-coated with 8-Isoprostane (15-Isoprostane F2t) antibody

8-Isoprostane (15-isoprostane F2t) Standard

5X Wash Buffer

5X Dilution Buffer

TMB Substrate

8-Isoprostane (15-isoprostane F2t) HRP Conjugate

Reagent Trough for multi-channel pipettor