NWLSS™ Superoxide Dismutase (SOD) Activity Assay Kit

推荐用于检测组织匀浆、细胞裂解物和其他可能存在超氧化物歧化酶(SOD)的生物液体或提取物中的超氧化物歧化酶(SOD)活性。若用KCN抑制胞质超氧化物歧化酶(Cu/Zn SOD)活性，则该试剂盒可以特异性检测线粒体超氧化物歧化酶(Mn SOD)的活性。

Superoxide radicals are involved in many physiological and pathophysiological processes. They are produced as a by-product of respiratory electron transport and cytochome P450 reactions. Activated neutrophils and macrophages can also produce a large amount during oxidative burst. Superoxide radical can react with NO at very fast rate to form peroxynitrite (ONOO-) a very powerful oxidant that has been shown to damage DNA, protein and other biological molecules. Removal of superoxide is a necessary step in cellular defense against these damages.

The enzyme superoxide dismutase (SOD) decomposes superoxide anion into hydrogen peroxide and oxygen at a high reaction rate. Since its initial discovery by McCord and Fridovich in 1969, SOD has been found to be ubiquitous in every aerobic organism from microbes to human.

Four types of SOD have been identified on the basis of their metal cofactors and distribution. The copper zinc form (Cu/ZnSOD) is most common with a primary distribution in the cytoplasm of eukaryotic cells. Another copper zinc form, extra-cellular (ecSOD), has more recently been identified. This SOD is often associated with vascular tissues but can also be found in plasma, lymph, synovial fluid, cerebrospinal fluid (CSF) and elsewhere. The manganese form (MnSOD) is generally associated with the mitochondria of aerobic organisms. The iron form (FeSOD) is found predominantly in prokaryotes.

The relationship between SOD and various human diseases has now been well documented and measurement of SOD activity has become commonplace in many research model systems. One unit SOD activity is defined as the amount of enzyme that will inhibit the rate of cytochrome c reduction by half under specific conditions.

格式:96 test酶标板或30比色皿

样品要求:组织匀浆，细胞裂解液或其他可能含有超氧化物歧化酶的生物样品。

特异性:cu /Zn、Mn、Fe超氧化物歧化酶

灵敏度:检测下限为5 U/mL SOD样品加入反应液中(反应液中0.2 U/mL)

用途:定量检测超氧化物歧化酶

储存和稳定性:储存在2-8℃，6个月。

Kit Contents:

Assay Buffer.............30 mL

Hematoxylin..............1 vial

Sample Dilution Buffer...30 mL

Transparent Microplate...1