谷胱甘肽S-转移酶荧光活性试剂盒
The DetectX® Glutathione S-Transferase (GST) Fluorescent Activity Kit is designed to quantitatively measure the activity of GST.

检测类型: Activity Assay
样本类型: Serum, Plasma (EDTA and Heparin), Urine, Cell Lysates
灵敏度: 2.70 mU/mL
适用物种: 人
检测时间: 30分钟
样品数/板: 40 in Duplicate
读数: Fluorescent, 460 nm emission / 370-410 nm excitation

检测原理:
The Glutathione S-Transferase (GST) Fluorescent Activity Kit quantitatively measures GST activity in serum, plasma (EDTA and Heparin), urine, and cell lysates. The Glutathione S-Transferase (GST) Fluorescent Activity Kit is an Activity Kit with a run time of 30 minutes. Please read the complete kit insert for more information before performing this assay.

Use our provided Glutathione S-Transferase Standard to generate a standard curve for the assay. Pipette the standards or diluted samples into a black microtiter plate. Add GST Detection Reagent and Glutathione (GSH) to each well tapping the plate to ensure sufficient mixing of reagents. Then incubate the mixture covered at room temperature for 30 minutes. The fluorescent reaction occurs between the GST Detection Reagent, the GSH, and the GST within the sample or standard.

After the 30-minute incubation, use a plate reader to detect and record the generated fluorescent signal at 460nm. Use the intensity and the standard curve to calculate the GST activity in the samples.

背景:
The Glutathione S-Transferase (GST) family of isozymes functions to detoxify and neutralize a wide variety of electrophilic molecules by mediating their conjugation with reduced glutathione. GST enzymes play a pivotal role in alleviating oxidative stress/damage. Several gene families encode Human GSTs, with expression in almost all tissues. GST enzymes are classified as either alpha, mu, pi, sigma, or theta, depending on their structure and substrate specificity. Scientists utilize GST activity as a biomarker for arthritis, asthma, and COPD. The acidic pi class of GSTs is highly prevalent in several human cancers. Furthermore, investigators hypothesize that its activity substantially contributes to the innate or acquired resistance of specific neoplasms to anticancer therapy.