DetectX®DHEA-S ELISA试剂盒用于检测多种物种的雌激素缺乏。

检测类型:

Competitive ELISA

样本类型:

Serum, Plasma, Urine, Saliva, Fecal Extracts, Tissue Culture Media

灵敏度:

90.9 pg/mL

适应物种:

DHEA-S在不同物种间相同

检测时间:

2.5小时

样品数/板:

41 in Duplicate

读数:

Colorimetric, 450 nm

DetectX®DHEA-S ELISA试剂盒使用特异性抗体来测定血清,血浆,尿液,唾液,粪便提取物,组织培养基样品中的DHEA-S。将标准品或稀释的样品吸移到包被抗体的微孔板中，以捕获绵羊抗体。将DHEA-S-过氧化物酶偶联物添加到孔中，通过向每个孔中加入DHEA-S多克隆抗体进行结合反应。孵育2小时后，洗涤板并加入底物，底物与结合的DHEA-S-过氧化物酶缀合物反应。在微量滴定板读数器中于450nm处检测产生的颜色的强度。

Dehydroepiandrosterone sulfate (DHEA-S) is the major C19 steroid secreted by the adrenal cortex, and is a precursor to testosterone and estrogen biosynthesis. It is produced by the addition of a sulfate group to dehydroepiandrosterone (DHEA) catalyzed by the sulfotransferase enzymes, SULT1A1 and SULT1E1, which also produce estrone sulfate from estrone. Due to the presence of a 17-ketone group rather than a hydroxyl group, DHEA-S has relatively low androgenic activity. The bioactivity of DHEA-S may be high due to its serum concentrations being 100-1,000 -fold higher than testosterone or DHEA in addition to its weak affinity for sex-hormone binding globulin.

Arbor Assays为临床重要生物分子研发最高质量的检测和免疫分析试剂盒,在生物领域建立健全的免疫检测和酶活性试剂盒以定量生物分子。Arbor Assays检测试剂盒具有高灵敏性,适用多物种及操作步骤简单的特点。
北京中昊新生科技作为美国Arbor Assays中国代理,一直为国内客户提供及时高效的配送和更专业的产品技术支持。为您提供DHEA-S ELISA试剂盒最新现货优惠信息。