孕酮ELISA试剂盒
DetectX®Progesterone ELISA试剂盒定量检测提取的干燥粪便样品,尿液和组织培养基样品中的孕酮。用于检测生殖和性类固醇代谢。

检测类型:Competitive ELISA

样本类型:Serum, Plasma, Urine, Fecal Extracts, Tissue Culture Media

灵敏度:47.9 pg/mL

适应物种:Progesterone在所有物种中都是相同的

检测时间:2.5小时

样品数/板:39 or 231 in Duplicate

读数:Colorimetric, 450 nm

DetectX®孕酮ELISA试剂盒提供有孕酮标准品以生成测定的标准曲线，将标准品或稀释的样品移入包被有抗体的透明微孔板中以捕获小鼠抗体。 将黄体酮-过氧化物酶结合物加入孔内， 通过向每个孔中加入孕酮单克隆抗体来开始结合反应。孵育2小时后，洗涤板并加入底物。 底物与结合的孕酮-过氧化物酶偶联物反应，短暂孵育后，停止反应，并在能够测量450nm波长的微量滴定板读取器中检测所产生颜色的强度。进行操作前请阅读完整试剂盒说明书或咨询中昊新生。

The Progesterone ELISA Kit quantitatively measures Progesterone in serum, plasma, fecal extracts, urine, and tissue culture media. The Progesterone ELISA Kit is a competitive ELISA with a run time of 2.5 hours. Please read the complete kit insert for more information before performing this assay.

Use our provided Progesterone standard to generate a standard curve for the assay. Pipette the standards or diluted samples into a transparent microtiter plate coated with our goat anti-mouse IgG antibody. Add the Progesterone peroxidase conjugate and the Progesterone monoclonal mouse antibody. Then incubate the mixture covered at room temperature, shaking for 2 hours. The immunological reaction occurs between the anti-Progesterone monoclonal antibody, the Progesterone antigen in the sample or standard, and the Progesterone-peroxidase conjugate. As the Progesterone concentration in the sample increases, the bound Progesterone-peroxidase conjugate decreases, causing a decrease in signal and vice versa. 

After the 2-hour incubation, wash away the excess Progesterone-peroxidase and add the TMB substrate. The TMB substrate reacts with the bound Progesterone-peroxidase conjugate generating a signal detected by a plate reader at 450nm. Use the intensity and the standard curve to calculate the Progesterone concentration in the samples.

背景:

Progesterone, also known as P4 (Pregn-4-ene-3,20-dione), is a C21 steroid hormone involved in the female menstrual cycle, gestation, and embryogenesis of humans and other species. It belongs to a class of hormones called progestogens and is the major naturally occurring progestogen.

Progesterone is an essential regulator of human female reproductive function in the uterus, ovary, mammary gland, and brain. It also plays vital roles in non-reproductive tissues such as the cardiovascular, skeletal, and central nervous systems. Two isoforms of the nuclear progesterone receptor (PR), PR-A and PR-B, dictate progesterone action. Various healthy and cancerous breast tissue cells from humans, rats, and mice express PR-A and PR-B. Progesterone also has neurotrophic roles in the peripheral nervous system. It activates the growth and maturation of axons and stimulates the repair and replacement of myelin sheaths in regenerating nerve fibers.

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