三碘甲状腺原氨酸ELISA试剂盒
适用多物种

Triiodothyronine (T3) ELISA试剂盒定量检测尿液,提取的血清,血浆,粪便提取物和组织培养基样品中存在的T3。它检测提取的血清,血浆和粪便提取物中的总T3。

检测类型:

Competitive ELISA

样本类型:

Urine, Tissue Culture Media, Extracted Serum, Plasma, and Fecal Extracts

灵敏度:

37.4 pg/mL

适应物种:

T3在不同物种间是相同的

检测时间:

2.5小时

样品数/板:

39 in Duplicate

读数:

Colorimetric, 450 nm

DetectX®三碘甲状腺原氨酸(T3) ELISA试剂盒用于定量测定尿液、提取的血清和血浆,提取的干燥粪便样品和组织培养液样品中存在的T3。该试剂盒可测量提取的血清和血浆以及提取的粪便样品中的总T3。提供T3储备液以生成测定的标准曲线。将标准品或稀释的样品吸移到微孔板中，将T3-过氧化物酶缀合物添加到孔中的标准品和样品中。通过向每个孔加入T3绵羊抗体来启动结合反应。孵育两小时后，洗涤板并加入底物。底物与结合的T3-过氧化物酶缀合物反应。孵育30分钟后，停止反应，在450nm处用微量滴定仪检测产生的颜色的强度。执行试剂盒操作前请阅读详细说明书或咨询中昊新生(zhscience.com)。

背景:

Triiodothyronine, also known as T3, is a thyroid hormone. Thyroid hormones regulate several developmental, metabolic, and neural activities throughout the body. T3 affects almost every physiological process in the body, including growth and development, metabolism, body temperature, and heart rate. Production of T3 and its prohormone, thyroxine (T4), is activated by thyroid-stimulating hormone (TSH) and released from the pituitary gland. This pathway is part of a closed-loop feedback process when elevated concentrations of T3 and T4 in the blood inhibit the production of TSH in the pituitary gland. As concentrations of these hormones decrease, the pituitary gland increases the production of TSH. Through these processes, a feedback control system stabilizes the number of thyroid hormones in 100 1.0 in the bloodstream. The concentration of serum T4 is 20 times that of T3.

Circulating levels of T4 are much more significant than T3 levels, but T3 is the more metabolically active hormone (3-4 times more potent than T4). However, its effect is briefer due to its shorter half-life. Elevated T3 and T4 are typical in patients with hyperthyroidism, but in a small subset of hyperthyroid patients, only T3 is elevated (T3 toxicosis). Triiodothyronine values greater than 2 ng/mL in adults or children are consistent with hyperthyroidism or high thyroid hormone-binding proteins. Diminished T3 and T4 are standard in patients with hypothyroidism, and T3 levels are frequently low in sick or hospitalized euthyroid patients.

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