NWLSS™ 丙二醛比色法(MDA)检测试剂盒 用于检测多种物种和样品类型中丙二醛(MDA)或硫巴比妥酸反应物质(TBARS)的比色检测试剂盒。这种分析方法设计简单，价格实惠，通过优化改进，保障了分析中最可靠的数据。

NWLSS™丙二醛检测的优点:

◆在样品和反应混合物中加入BHT和EDTA，以最大限度地减少假象氧化。

◆降低的反应温度使脂质氢过氧化物的分解最小化。

◆优化的反应pH有利于MDA-蛋白加合物的水解，从而更好地回收丙二醛(MDA)。

◆通过使用双波长或光谱扫描数据的三阶导数分析优化数据缩减，使得输出更清晰。

导数光谱法检测方案.PDF

单点TBARs检测方案.PDF

Lipid peroxidation has been established as a major mechanism of cellular injury in many biological systems of plant and animal origin. The mechanism involves a process whereby unsaturated lipids are oxidized to form additional radical species as well as toxic by-products that can be harmful to the host system. Polyunsaturated lipids are especially susceptible to this type of damage when in an oxidizing environment and they can react to form lipid peroxides.

Lipid peroxides are themselves unstable, and undergo aditional decomposition to form a complex series of compounds including reactive carbonyl compounds. Polyunsaturated fatty acid peroxides further react to form malonaldehyde (MDA).

MDA can be found in most biological samples including foodstuffs, serum, plasma, tissues and urine, as a result of lipid peroxidation, and has become one of the most widely reported analytes for the purpose of estimating oxidative stress effects on lipids.

This assay is based on the reaction of malondialdehyde (MDA) with thiobarbituric acid (TBA); forming a MDA-TBA2 adduct that absorbs strongly at 532 nm.

This reaction is the most popular method for estimating MDA in biological samples. However, interference can be a significant problem in some biological samples if not dealt with appropriately. Our assay provides the most accurate and efficient means of dealing with elevated backgrounds commonly associated with the TBARS reaction.

格式：基于TBA的比色法

样品要求：组织匀浆、细胞裂解物和血浆

特异性：当使用先进的数据缩减和/或反萃取技术时，主要特异性是针对丙二醛 (MDA)。 进行单波长 532nm 测量时检测到碱性硫代巴比妥酸反应物质 (TBARS)。

灵敏度：样品MDA为0.1 µM

检测范围：1.0 - 4.0 mM

储存和稳定性：在2-8℃储存时，自制造之日起 12 个月

Kit Contents:

5 X TBA in powder form

5 X H3PO4; ready to use

5 X TMOP Calibrators; ready to use

1 x Butylated Hydroxytoluene (BHT; ready to use)

1 x Assay Buffer